Construct: shRNA TRCN0000199760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014975.1-3145s1c1
DNA Barcode:
GTCGTGGAGCTGATCCTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST1 (22983)
Gene Description:
microtubule associated serine/threonine kinase 1
Transcript:
RefSeq NM_014975.1 (NON-CURRENT)
Match location:
Position 3145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGTGGAGCTGATCCTTAAG
Hairpin Sequence:
5'-CCGG-GTCGTGGAGCTGATCCTTAAG-CTCGAG-CTTAAGGATCAGCTCCACGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGTGGAGCTGATCCTTAAGCTCGAGCTTAAGGATCAGCTCCACGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGTGGAGCTGATCCTTAAGCTCGAGCTTAAGGATCAGCTCCACGAC-3'

Other clones with same target sequence:

(none)