Construct: shRNA TRCN0000199783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004614.3-568s1c1
DNA Barcode:
GCGACAGACGTCGAGGTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TK2 (7084)
Gene Description:
thymidine kinase 2
Transcript:
RefSeq NM_004614.3 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACAGACGTCGAGGTGTTA
Hairpin Sequence:
5'-CCGG-GCGACAGACGTCGAGGTGTTA-CTCGAG-TAACACCTCGACGTCTGTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACAGACGTCGAGGTGTTACTCGAGTAACACCTCGACGTCTGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACAGACGTCGAGGTGTTACTCGAGTAACACCTCGACGTCTGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000350979