Construct: shRNA TRCN0000199786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014370.2-1867s1c1
DNA Barcode:
GAGCGTGTTCTGCCTGAGACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRPK3 (26576)
Gene Description:
SRSF protein kinase 3
Transcript:
RefSeq NM_014370.2 (NON-CURRENT)
Match location:
Position 1867 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGTGTTCTGCCTGAGACC
Hairpin Sequence:
5'-CCGG-GAGCGTGTTCTGCCTGAGACC-CTCGAG-GGTCTCAGGCAGAACACGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGTGTTCTGCCTGAGACCCTCGAGGGTCTCAGGCAGAACACGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGTGTTCTGCCTGAGACCCTCGAGGGTCTCAGGCAGAACACGCTC-3'

Other clones with same target sequence:

(none)