Construct: shRNA TRCN0000199792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291277.5-2064s1c1
DNA Barcode:
GATGAGCAAATCCGCCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRAG1 (157285)
Gene Description:
PEAK1 related, kinase-activating pseudokinase 1
Transcript:
RefSeq XM_291277.5 (NON-CURRENT)
Match location:
Position 2064 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGCAAATCCGCCTCTTT
Hairpin Sequence:
5'-CCGG-GATGAGCAAATCCGCCTCTTT-CTCGAG-AAAGAGGCGGATTTGCTCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGCAAATCCGCCTCTTTCTCGAGAAAGAGGCGGATTTGCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGCAAATCCGCCTCTTTCTCGAGAAAGAGGCGGATTTGCTCATC-3'

Other clones with same target sequence:

(none)