Construct: shRNA TRCN0000199802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005027.2-2090s1c1
DNA Barcode:
GCGGCTCAAGTCCCGCATTGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3R2 (5296)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 2
Transcript:
RefSeq NM_005027.2 (NON-CURRENT)
Match location:
Position 2090 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTCAAGTCCCGCATTGC
Hairpin Sequence:
5'-CCGG-GCGGCTCAAGTCCCGCATTGC-CTCGAG-GCAATGCGGGACTTGAGCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTCAAGTCCCGCATTGCCTCGAGGCAATGCGGGACTTGAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTCAAGTCCCGCATTGCCTCGAGGCAATGCGGGACTTGAGCCGC-3'

Other clones with same target sequence:

(none)