Construct: shRNA TRCN0000199813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001106.2-1139s1c1
DNA Barcode:
GATGCCTTCCTGCGCATTGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR2B (93)
Gene Description:
activin A receptor type 2B
Transcript:
RefSeq NM_001106.2 (NON-CURRENT)
Match location:
Position 1139 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCCTTCCTGCGCATTGAC
Hairpin Sequence:
5'-CCGG-GATGCCTTCCTGCGCATTGAC-CTCGAG-GTCAATGCGCAGGAAGGCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCCTTCCTGCGCATTGACCTCGAGGTCAATGCGCAGGAAGGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCCTTCCTGCGCATTGACCTCGAGGTCAATGCGCAGGAAGGCATC-3'

Other clones with same target sequence:

  1. TRCN0000318687