Construct: shRNA TRCN0000199818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002760.3-566s1c1
DNA Barcode:
GCCCTCAAGGTGATGAGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKY (5616)
Gene Description:
protein kinase Y-linked (pseudogene)
Transcript:
RefSeq NM_002760.3 (NON-CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCAAGGTGATGAGCATT
Hairpin Sequence:
5'-CCGG-GCCCTCAAGGTGATGAGCATT-CTCGAG-AATGCTCATCACCTTGAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCAAGGTGATGAGCATTCTCGAGAATGCTCATCACCTTGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCAAGGTGATGAGCATTCTCGAGAATGCTCATCACCTTGAGGGC-3'

Other clones with same target sequence:

(none)