Construct: shRNA TRCN0000199852
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004567.2-1211s1c1
- DNA Barcode:
- GCTGGCCTACTTCCTCGACAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PFKFB4 (5210)
- Gene Description:
- 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
- Transcript:
- RefSeq NM_004567.2 (NON-CURRENT)
- Match location:
- Position 1211 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000199852)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCTGGCCTACTTCCTCGACAA
- Hairpin Sequence:
- 5'-CCGG-GCTGGCCTACTTCCTCGACAA-CTCGAG-TTGTCGAGGAAGTAGGCCAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGGCCTACTTCCTCGACAACTCGAGTTGTCGAGGAAGTAGGCCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGGCCTACTTCCTCGACAACTCGAGTTGTCGAGGAAGTAGGCCAGC-3'