Construct: shRNA TRCN0000199909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004567.2-3341s1c1
DNA Barcode:
GCTGATTGGCTGCCACATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKFB4 (5210)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Transcript:
RefSeq NM_004567.2 (NON-CURRENT)
Match location:
Position 3341 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATTGGCTGCCACATTTC
Hairpin Sequence:
5'-CCGG-GCTGATTGGCTGCCACATTTC-CTCGAG-GAAATGTGGCAGCCAATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATTGGCTGCCACATTTCCTCGAGGAAATGTGGCAGCCAATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATTGGCTGCCACATTTCCTCGAGGAAATGTGGCAGCCAATCAGC-3'

Other clones with same target sequence:

(none)