Construct: shRNA TRCN0000199992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001654.1-2224s1c1
DNA Barcode:
CCTCCATCATTTGGTTTCCTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARAF (369)
Gene Description:
A-Raf proto-oncogene, serine/threonine kinase
Transcript:
RefSeq NM_001654.1 (NON-CURRENT)
Match location:
Position 2224 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCATCATTTGGTTTCCTC
Hairpin Sequence:
5'-CCGG-CCTCCATCATTTGGTTTCCTC-CTCGAG-GAGGAAACCAAATGATGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCATCATTTGGTTTCCTCCTCGAGGAGGAAACCAAATGATGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCATCATTTGGTTTCCTCCTCGAGGAGGAAACCAAATGATGGAGG-3'

Other clones with same target sequence:

(none)