Construct: shRNA TRCN0000200045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172801.1-1658s1c1
DNA Barcode:
CCAAGGCATCACCTTTGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otop2 (237987)
Gene Description:
otopetrin 2
Transcript:
RefSeq NM_172801.1 (NON-CURRENT)
Match location:
Position 1658 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGCATCACCTTTGCTAA
Hairpin Sequence:
5'-CCGG-CCAAGGCATCACCTTTGCTAA-CTCGAG-TTAGCAAAGGTGATGCCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGCATCACCTTTGCTAACTCGAGTTAGCAAAGGTGATGCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGCATCACCTTTGCTAACTCGAGTTAGCAAAGGTGATGCCTTGG-3'

Other clones with same target sequence:

(none)