Construct: shRNA TRCN0000200083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026797.1-766s1c1
DNA Barcode:
CGCAGTCCAAATCCAAGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dbndd2 (52840)
Gene Description:
dysbindin (dystrobrevin binding protein 1) domain containing 2
Transcript:
RefSeq NM_026797.1 (NON-CURRENT)
Match location:
Position 766 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGTCCAAATCCAAGTGAT
Hairpin Sequence:
5'-CCGG-CGCAGTCCAAATCCAAGTGAT-CTCGAG-ATCACTTGGATTTGGACTGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGTCCAAATCCAAGTGATCTCGAGATCACTTGGATTTGGACTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGTCCAAATCCAAGTGATCTCGAGATCACTTGGATTTGGACTGCG-3'

Other clones with same target sequence:

(none)