Construct: shRNA TRCN0000200086
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030026.1-1108s1c1
- DNA Barcode:
- CCGAGAGGTCATTGCTAGAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mccc2 (78038)
- Gene Description:
- methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
- Transcript:
- RefSeq NM_030026.1 (NON-CURRENT)
- Match location:
- Position 1108 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000200086)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGAGAGGTCATTGCTAGAAT
- Hairpin Sequence:
-
5'-CCGG-CCGAGAGGTCATTGCTAGAAT-CTCGAG-ATTCTAGCAATGACCTCTCGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGAGAGGTCATTGCTAGAATCTCGAGATTCTAGCAATGACCTCTCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGAGAGGTCATTGCTAGAATCTCGAGATTCTAGCAATGACCTCTCGG-3'
Other clones with same target sequence:
-
TRCN0000298148
