Construct: shRNA TRCN0000200134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174994.1-418s1c1
DNA Barcode:
CTACAGGAAGACCAACAGAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC016548 (211039)
Gene Description:
cDNA sequence BC016548
Transcript:
RefSeq NM_174994.1 (NON-CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAGGAAGACCAACAGAGT
Hairpin Sequence:
5'-CCGG-CTACAGGAAGACCAACAGAGT-CTCGAG-ACTCTGTTGGTCTTCCTGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAGGAAGACCAACAGAGTCTCGAGACTCTGTTGGTCTTCCTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAGGAAGACCAACAGAGTCTCGAGACTCTGTTGGTCTTCCTGTAG-3'

Other clones with same target sequence:

(none)