Construct: shRNA TRCN0000200166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029847.2-1373s1c1
DNA Barcode:
CCAGTGGAAGTACATAGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arsk (77041)
Gene Description:
arylsulfatase K
Transcript:
RefSeq NM_029847.2 (NON-CURRENT)
Match location:
Position 1373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGGAAGTACATAGCCTA
Hairpin Sequence:
5'-CCGG-CCAGTGGAAGTACATAGCCTA-CTCGAG-TAGGCTATGTACTTCCACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGGAAGTACATAGCCTACTCGAGTAGGCTATGTACTTCCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGGAAGTACATAGCCTACTCGAGTAGGCTATGTACTTCCACTGG-3'

Other clones with same target sequence:

(none)