Construct: shRNA TRCN0000200198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021447.1-368s1c1
DNA Barcode:
CCTCTAATCCTCTGTGGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim54 (58522)
Gene Description:
tripartite motif-containing 54
Transcript:
RefSeq NM_021447.1 (NON-CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTAATCCTCTGTGGCAAT
Hairpin Sequence:
5'-CCGG-CCTCTAATCCTCTGTGGCAAT-CTCGAG-ATTGCCACAGAGGATTAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTAATCCTCTGTGGCAATCTCGAGATTGCCACAGAGGATTAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTAATCCTCTGTGGCAATCTCGAGATTGCCACAGAGGATTAGAGG-3'

Other clones with same target sequence:

(none)