Construct: shRNA TRCN0000200240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007825.1-1621s1c1
DNA Barcode:
CTTTGCAGAGTAAGGCTGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp7b1 (13123)
Gene Description:
cytochrome P450, family 7, subfamily b, polypeptide 1
Transcript:
RefSeq NM_007825.1 (NON-CURRENT)
Match location:
Position 1621 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCAGAGTAAGGCTGCAT
Hairpin Sequence:
5'-CCGG-CTTTGCAGAGTAAGGCTGCAT-CTCGAG-ATGCAGCCTTACTCTGCAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCAGAGTAAGGCTGCATCTCGAGATGCAGCCTTACTCTGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCAGAGTAAGGCTGCATCTCGAGATGCAGCCTTACTCTGCAAAG-3'

Other clones with same target sequence:

(none)