Construct: shRNA TRCN0000200346

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207217.1-774s1c1
DNA Barcode:
GCAGCTTTAGTGGGAACGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam234a (106581)
Gene Description:
family with sequence similarity 234, member A
Transcript:
RefSeq NM_207217.1 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200346)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTTAGTGGGAACGTTT
Hairpin Sequence:
5'-CCGG-GCAGCTTTAGTGGGAACGTTT-CTCGAG-AAACGTTCCCACTAAAGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTTAGTGGGAACGTTTCTCGAGAAACGTTCCCACTAAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTTAGTGGGAACGTTTCTCGAGAAACGTTCCCACTAAAGCTGC-3'

Other clones with same target sequence:

(none)