Construct: shRNA TRCN0000200428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194339.1-1682s1c1
DNA Barcode:
GCGTTTGCTGATAGCGATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bms1 (213895)
Gene Description:
BMS1, ribosome biogenesis factor
Transcript:
RefSeq NM_194339.1 (CURRENT)
Match location:
Position 1682 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTTGCTGATAGCGATGAT
Hairpin Sequence:
5'-CCGG-GCGTTTGCTGATAGCGATGAT-CTCGAG-ATCATCGCTATCAGCAAACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTTGCTGATAGCGATGATCTCGAGATCATCGCTATCAGCAAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTTGCTGATAGCGATGATCTCGAGATCATCGCTATCAGCAAACGC-3'

Other clones with same target sequence:

  1. TRCN0000279433