Construct: shRNA TRCN0000200495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029394.2-1566s1c1
DNA Barcode:
CGTATGGATTAAAGTGAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx24 (69226)
Gene Description:
sorting nexing 24
Transcript:
RefSeq NM_029394.2 (NON-CURRENT)
Match location:
Position 1566 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATGGATTAAAGTGAGTTA
Hairpin Sequence:
5'-CCGG-CGTATGGATTAAAGTGAGTTA-CTCGAG-TAACTCACTTTAATCCATACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATGGATTAAAGTGAGTTACTCGAGTAACTCACTTTAATCCATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATGGATTAAAGTGAGTTACTCGAGTAACTCACTTTAATCCATACG-3'

Other clones with same target sequence:

(none)