Construct: shRNA TRCN0000200542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177865.2-443s1c1
DNA Barcode:
CTCAGTCATGTACATTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Skint2 (329919)
Gene Description:
selection and upkeep of intraepithelial T cells 2
Transcript:
RefSeq NM_177865.2 (NON-CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGTCATGTACATTCTAAT
Hairpin Sequence:
5'-CCGG-CTCAGTCATGTACATTCTAAT-CTCGAG-ATTAGAATGTACATGACTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGTCATGTACATTCTAATCTCGAGATTAGAATGTACATGACTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGTCATGTACATTCTAATCTCGAGATTAGAATGTACATGACTGAG-3'

Other clones with same target sequence:

(none)