Construct: shRNA TRCN0000200598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017362.1-570s1c1
DNA Barcode:
CATGAAGTATTTGTACCCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arid3c (550619)
Gene Description:
AT rich interactive domain 3C (BRIGHT-like)
Transcript:
RefSeq NM_001017362.1 (NON-CURRENT)
Match location:
Position 570 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAAGTATTTGTACCCATA
Hairpin Sequence:
5'-CCGG-CATGAAGTATTTGTACCCATA-CTCGAG-TATGGGTACAAATACTTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAAGTATTTGTACCCATACTCGAGTATGGGTACAAATACTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAAGTATTTGTACCCATACTCGAGTATGGGTACAAATACTTCATG-3'

Other clones with same target sequence:

(none)