Construct: shRNA TRCN0000200617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025681.1-975s1c1
DNA Barcode:
CTGACAGAAGTTGTTAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lix1 (66643)
Gene Description:
limb and CNS expressed 1
Transcript:
RefSeq NM_025681.1 (NON-CURRENT)
Match location:
Position 975 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACAGAAGTTGTTAGAGAT
Hairpin Sequence:
5'-CCGG-CTGACAGAAGTTGTTAGAGAT-CTCGAG-ATCTCTAACAACTTCTGTCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACAGAAGTTGTTAGAGATCTCGAGATCTCTAACAACTTCTGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACAGAAGTTGTTAGAGATCTCGAGATCTCTAACAACTTCTGTCAG-3'

Other clones with same target sequence:

(none)