Construct: shRNA TRCN0000200621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153179.2-6533s1c1
DNA Barcode:
CCTGAGATATTCCTATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkhd1 (241035)
Gene Description:
polycystic kidney and hepatic disease 1
Transcript:
RefSeq NM_153179.2 (NON-CURRENT)
Match location:
Position 6533 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGATATTCCTATGATTT
Hairpin Sequence:
5'-CCGG-CCTGAGATATTCCTATGATTT-CTCGAG-AAATCATAGGAATATCTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGATATTCCTATGATTTCTCGAGAAATCATAGGAATATCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGATATTCCTATGATTTCTCGAGAAATCATAGGAATATCTCAGG-3'

Other clones with same target sequence:

(none)