Construct: shRNA TRCN0000200685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175937.2-1164s1c1
DNA Barcode:
GTCCTTGGAATTTAAGTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpeb2 (231207)
Gene Description:
cytoplasmic polyadenylation element binding protein 2
Transcript:
RefSeq NM_175937.2 (NON-CURRENT)
Match location:
Position 1164 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCTTGGAATTTAAGTGATA
Hairpin Sequence:
5'-CCGG-GTCCTTGGAATTTAAGTGATA-CTCGAG-TATCACTTAAATTCCAAGGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCTTGGAATTTAAGTGATACTCGAGTATCACTTAAATTCCAAGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCTTGGAATTTAAGTGATACTCGAGTATCACTTAAATTCCAAGGAC-3'

Other clones with same target sequence:

(none)