Construct: shRNA TRCN0000200735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172510.1-1247s1c1
DNA Barcode:
GCCTGACACTTTAGTTATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mfsd4a (213006)
Gene Description:
major facilitator superfamily domain containing 4A
Transcript:
RefSeq NM_172510.1 (NON-CURRENT)
Match location:
Position 1247 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGACACTTTAGTTATCTT
Hairpin Sequence:
5'-CCGG-GCCTGACACTTTAGTTATCTT-CTCGAG-AAGATAACTAAAGTGTCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGACACTTTAGTTATCTTCTCGAGAAGATAACTAAAGTGTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGACACTTTAGTTATCTTCTCGAGAAGATAACTAAAGTGTCAGGC-3'

Other clones with same target sequence:

(none)