Construct: shRNA TRCN0000200736
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175147.2-285s1c1
- DNA Barcode:
- GATCTATTTGATTGACAGGCA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Etd (69501)
- Gene Description:
- embryonic testis differentiation
- Transcript:
- RefSeq NM_175147.2 (NON-CURRENT)
- Match location:
- Position 285 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000200736)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GATCTATTTGATTGACAGGCA
- Hairpin Sequence:
-
5'-CCGG-GATCTATTTGATTGACAGGCA-CTCGAG-TGCCTGTCAATCAAATAGATC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGATCTATTTGATTGACAGGCACTCGAGTGCCTGTCAATCAAATAGATCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGATCTATTTGATTGACAGGCACTCGAGTGCCTGTCAATCAAATAGATC-3'
Other clones with same target sequence:
(none)
