Construct: shRNA TRCN0000200829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026082.2-2684s1c1
DNA Barcode:
GCCTTTGTTTCAAGGACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dock7 (67299)
Gene Description:
dedicator of cytokinesis 7
Transcript:
RefSeq NM_026082.2 (NON-CURRENT)
Match location:
Position 2684 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGTTTCAAGGACTTTA
Hairpin Sequence:
5'-CCGG-GCCTTTGTTTCAAGGACTTTA-CTCGAG-TAAAGTCCTTGAAACAAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGTTTCAAGGACTTTACTCGAGTAAAGTCCTTGAAACAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGTTTCAAGGACTTTACTCGAGTAAAGTCCTTGAAACAAAGGC-3'

Other clones with same target sequence:

(none)