Construct: shRNA TRCN0000200871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026474.2-505s1c1
DNA Barcode:
GCCAAGAAATTCAGAATGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sugt1 (67955)
Gene Description:
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Transcript:
RefSeq NM_026474.2 (NON-CURRENT)
Match location:
Position 505 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAAATTCAGAATGGAT
Hairpin Sequence:
5'-CCGG-GCCAAGAAATTCAGAATGGAT-CTCGAG-ATCCATTCTGAATTTCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAAATTCAGAATGGATCTCGAGATCCATTCTGAATTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAAATTCAGAATGGATCTCGAGATCCATTCTGAATTTCTTGGC-3'

Other clones with same target sequence:

(none)