Construct: shRNA TRCN0000200918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028018.1-157s1c1
DNA Barcode:
CGTCTCAAACTGTTGGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ist1 (71955)
Gene Description:
increased sodium tolerance 1 homolog (yeast)
Transcript:
RefSeq NM_028018.1 (NON-CURRENT)
Match location:
Position 157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTCAAACTGTTGGAGAAA
Hairpin Sequence:
5'-CCGG-CGTCTCAAACTGTTGGAGAAA-CTCGAG-TTTCTCCAACAGTTTGAGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTCAAACTGTTGGAGAAACTCGAGTTTCTCCAACAGTTTGAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTCAAACTGTTGGAGAAACTCGAGTTTCTCCAACAGTTTGAGACG-3'

Other clones with same target sequence:

(none)