Construct: shRNA TRCN0000200949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183172.1-459s1c1
DNA Barcode:
CCCAGTTATTGTGGAATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ric8b (237422)
Gene Description:
RIC8 guanine nucleotide exchange factor B
Transcript:
RefSeq NM_183172.1 (NON-CURRENT)
Match location:
Position 459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTTATTGTGGAATCATT
Hairpin Sequence:
5'-CCGG-CCCAGTTATTGTGGAATCATT-CTCGAG-AATGATTCCACAATAACTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTTATTGTGGAATCATTCTCGAGAATGATTCCACAATAACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTTATTGTGGAATCATTCTCGAGAATGATTCCACAATAACTGGG-3'

Other clones with same target sequence:

(none)