Construct: shRNA TRCN0000200955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026236.1-1977s1c1
DNA Barcode:
CGGACAGTAAAGCACTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr48 (67561)
Gene Description:
WD repeat domain 48
Transcript:
RefSeq NM_026236.1 (NON-CURRENT)
Match location:
Position 1977 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACAGTAAAGCACTTCATA
Hairpin Sequence:
5'-CCGG-CGGACAGTAAAGCACTTCATA-CTCGAG-TATGAAGTGCTTTACTGTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACAGTAAAGCACTTCATACTCGAGTATGAAGTGCTTTACTGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACAGTAAAGCACTTCATACTCGAGTATGAAGTGCTTTACTGTCCG-3'

Other clones with same target sequence:

  1. TRCN0000328312