Construct: shRNA TRCN0000200960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146112.2-323s1c1
DNA Barcode:
CCCTTTGATCTTCTGGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gigyf2 (227331)
Gene Description:
GRB10 interacting GYF protein 2
Transcript:
RefSeq NM_146112.2 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGATCTTCTGGAGAAA
Hairpin Sequence:
5'-CCGG-CCCTTTGATCTTCTGGAGAAA-CTCGAG-TTTCTCCAGAAGATCAAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGATCTTCTGGAGAAACTCGAGTTTCTCCAGAAGATCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGATCTTCTGGAGAAACTCGAGTTTCTCCAGAAGATCAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000277090