Construct: shRNA TRCN0000200961

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172293.2-1594s1c1
DNA Barcode:
CTCTATCCTCAAGTTTCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pced1b (239647)
Gene Description:
PC-esterase domain containing 1B
Transcript:
RefSeq NM_172293.2 (NON-CURRENT)
Match location:
Position 1594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200961)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTATCCTCAAGTTTCCTAT
Hairpin Sequence:
5'-CCGG-CTCTATCCTCAAGTTTCCTAT-CTCGAG-ATAGGAAACTTGAGGATAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTATCCTCAAGTTTCCTATCTCGAGATAGGAAACTTGAGGATAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTATCCTCAAGTTTCCTATCTCGAGATAGGAAACTTGAGGATAGAG-3'

Other clones with same target sequence:

(none)