Construct: shRNA TRCN0000200981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173753.3-2363s1c1
DNA Barcode:
CGTTCTTGATTGGAGATTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fnip1 (216742)
Gene Description:
folliculin interacting protein 1
Transcript:
RefSeq NM_173753.3 (NON-CURRENT)
Match location:
Position 2363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000200981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCTTGATTGGAGATTCTA
Hairpin Sequence:
5'-CCGG-CGTTCTTGATTGGAGATTCTA-CTCGAG-TAGAATCTCCAATCAAGAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCTTGATTGGAGATTCTACTCGAGTAGAATCTCCAATCAAGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCTTGATTGGAGATTCTACTCGAGTAGAATCTCCAATCAAGAACG-3'

Other clones with same target sequence:

(none)