Construct: shRNA TRCN0000201011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178373.2-1507s1c1
DNA Barcode:
CCCTCCTGTAAGTAACCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cidec (14311)
Gene Description:
cell death-inducing DFFA-like effector c
Transcript:
RefSeq NM_178373.2 (NON-CURRENT)
Match location:
Position 1507 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCTGTAAGTAACCTAAA
Hairpin Sequence:
5'-CCGG-CCCTCCTGTAAGTAACCTAAA-CTCGAG-TTTAGGTTACTTACAGGAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCTGTAAGTAACCTAAACTCGAGTTTAGGTTACTTACAGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCTGTAAGTAACCTAAACTCGAGTTTAGGTTACTTACAGGAGGG-3'

Other clones with same target sequence:

(none)