Construct: shRNA TRCN0000201154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183166.1-1152s1c1
DNA Barcode:
CAGTCAAGGAACAGAGTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4884 (233164)
Gene Description:
predicted gene 4884
Transcript:
RefSeq NM_183166.1 (NON-CURRENT)
Match location:
Position 1152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTCAAGGAACAGAGTCAAA
Hairpin Sequence:
5'-CCGG-CAGTCAAGGAACAGAGTCAAA-CTCGAG-TTTGACTCTGTTCCTTGACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTCAAGGAACAGAGTCAAACTCGAGTTTGACTCTGTTCCTTGACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTCAAGGAACAGAGTCAAACTCGAGTTTGACTCTGTTCCTTGACTG-3'

Other clones with same target sequence:

(none)