Construct: shRNA TRCN0000201191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145967.1-521s1c1
DNA Barcode:
GACCAAGATTAGTACAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vstm2a (211739)
Gene Description:
V-set and transmembrane domain containing 2A
Transcript:
RefSeq NM_145967.1 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAAGATTAGTACAGTGAA
Hairpin Sequence:
5'-CCGG-GACCAAGATTAGTACAGTGAA-CTCGAG-TTCACTGTACTAATCTTGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAAGATTAGTACAGTGAACTCGAGTTCACTGTACTAATCTTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAAGATTAGTACAGTGAACTCGAGTTCACTGTACTAATCTTGGTC-3'

Other clones with same target sequence:

(none)