Construct: shRNA TRCN0000201193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152817.2-1109s1c1
DNA Barcode:
GCCAAGAATCTTGAGCTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc27 (74196)
Gene Description:
tetratricopeptide repeat domain 27
Transcript:
RefSeq NM_152817.2 (NON-CURRENT)
Match location:
Position 1109 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAATCTTGAGCTCAAT
Hairpin Sequence:
5'-CCGG-GCCAAGAATCTTGAGCTCAAT-CTCGAG-ATTGAGCTCAAGATTCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAATCTTGAGCTCAATCTCGAGATTGAGCTCAAGATTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAATCTTGAGCTCAATCTCGAGATTGAGCTCAAGATTCTTGGC-3'

Other clones with same target sequence:

(none)