Construct: shRNA TRCN0000201218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026879.1-446s1c1
DNA Barcode:
GTCCACACAAACGAAAGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp2b (68942)
Gene Description:
charged multivesicular body protein 2B
Transcript:
RefSeq NM_026879.1 (NON-CURRENT)
Match location:
Position 446 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCACACAAACGAAAGTGAT
Hairpin Sequence:
5'-CCGG-GTCCACACAAACGAAAGTGAT-CTCGAG-ATCACTTTCGTTTGTGTGGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCACACAAACGAAAGTGATCTCGAGATCACTTTCGTTTGTGTGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCACACAAACGAAAGTGATCTCGAGATCACTTTCGTTTGTGTGGAC-3'

Other clones with same target sequence:

(none)