Construct: shRNA TRCN0000201304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173189.2-718s1c1
DNA Barcode:
GCAGTGAATACCGATGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcph1 (244329)
Gene Description:
microcephaly, primary autosomal recessive 1
Transcript:
RefSeq NM_173189.2 (CURRENT)
Match location:
Position 718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGAATACCGATGAACAT
Hairpin Sequence:
5'-CCGG-GCAGTGAATACCGATGAACAT-CTCGAG-ATGTTCATCGGTATTCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGAATACCGATGAACATCTCGAGATGTTCATCGGTATTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGAATACCGATGAACATCTCGAGATGTTCATCGGTATTCACTGC-3'

Other clones with same target sequence:

(none)