Construct: shRNA TRCN0000201307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007784.2-1148s1c1
DNA Barcode:
GCCAATGATTCATCTTGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csn1s1 (12990)
Gene Description:
casein alpha s1
Transcript:
RefSeq NM_007784.2 (NON-CURRENT)
Match location:
Position 1148 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGATTCATCTTGAGTT
Hairpin Sequence:
5'-CCGG-GCCAATGATTCATCTTGAGTT-CTCGAG-AACTCAAGATGAATCATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGATTCATCTTGAGTTCTCGAGAACTCAAGATGAATCATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGATTCATCTTGAGTTCTCGAGAACTCAAGATGAATCATTGGC-3'

Other clones with same target sequence:

(none)