Construct: shRNA TRCN0000201326
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025434.2-597s1c1
- DNA Barcode:
- GCTTGCTGTATTTGAACCCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mrps28 (66230)
- Gene Description:
- mitochondrial ribosomal protein S28
- Transcript:
- RefSeq NM_025434.2 (NON-CURRENT)
- Match location:
- Position 597 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000201326)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTGCTGTATTTGAACCCAT
- Hairpin Sequence:
-
5'-CCGG-GCTTGCTGTATTTGAACCCAT-CTCGAG-ATGGGTTCAAATACAGCAAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTGCTGTATTTGAACCCATCTCGAGATGGGTTCAAATACAGCAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTGCTGTATTTGAACCCATCTCGAGATGGGTTCAAATACAGCAAGC-3'
Other clones with same target sequence:
(none)
