Construct: shRNA TRCN0000201342

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033312.1-2434s1c1
DNA Barcode:
GAGTACAGTGACAAGAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl18 (231863)
Gene Description:
F-box and leucine-rich repeat protein 18
Transcript:
RefSeq NM_001033312.1 (NON-CURRENT)
Match location:
Position 2434 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201342)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTACAGTGACAAGAAGTAA
Hairpin Sequence:
5'-CCGG-GAGTACAGTGACAAGAAGTAA-CTCGAG-TTACTTCTTGTCACTGTACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTACAGTGACAAGAAGTAACTCGAGTTACTTCTTGTCACTGTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTACAGTGACAAGAAGTAACTCGAGTTACTTCTTGTCACTGTACTC-3'

Other clones with same target sequence:

(none)