Construct: shRNA TRCN0000201390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198642.1-2328s1c1
DNA Barcode:
GCCCTCTTGTACGCATTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rubcnl (271221)
Gene Description:
RUN and cysteine rich domain containing beclin 1 interacting protein like
Transcript:
RefSeq NM_198642.1 (NON-CURRENT)
Match location:
Position 2328 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCTTGTACGCATTTAAT
Hairpin Sequence:
5'-CCGG-GCCCTCTTGTACGCATTTAAT-CTCGAG-ATTAAATGCGTACAAGAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCTTGTACGCATTTAATCTCGAGATTAAATGCGTACAAGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCTTGTACGCATTTAATCTCGAGATTAAATGCGTACAAGAGGGC-3'

Other clones with same target sequence:

(none)