Construct: shRNA TRCN0000201437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198671.1-3637s1c1
DNA Barcode:
CAAGGGATTGAGGCAATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gse1 (382034)
Gene Description:
genetic suppressor element 1, coiled-coil protein
Transcript:
RefSeq NM_198671.1 (NON-CURRENT)
Match location:
Position 3637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGGATTGAGGCAATCTTT
Hairpin Sequence:
5'-CCGG-CAAGGGATTGAGGCAATCTTT-CTCGAG-AAAGATTGCCTCAATCCCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGGATTGAGGCAATCTTTCTCGAGAAAGATTGCCTCAATCCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGGATTGAGGCAATCTTTCTCGAGAAAGATTGCCTCAATCCCTTG-3'

Other clones with same target sequence:

(none)