Construct: shRNA TRCN0000201497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026412.1-968s1c1
DNA Barcode:
CCTTATGTAAGGACGAAGCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Knstrn (51944)
Gene Description:
kinetochore-localized astrin/SPAG5 binding
Transcript:
RefSeq NM_026412.1 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTATGTAAGGACGAAGCCA
Hairpin Sequence:
5'-CCGG-CCTTATGTAAGGACGAAGCCA-CTCGAG-TGGCTTCGTCCTTACATAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTATGTAAGGACGAAGCCACTCGAGTGGCTTCGTCCTTACATAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTATGTAAGGACGAAGCCACTCGAGTGGCTTCGTCCTTACATAAGG-3'

Other clones with same target sequence:

  1. TRCN0000319897