Construct: shRNA TRCN0000201521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010750.2-1209s1c1
DNA Barcode:
CAGGAAGAAATGCCTCTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mab21l1 (17116)
Gene Description:
mab-21-like 1 (C. elegans)
Transcript:
RefSeq NM_010750.2 (NON-CURRENT)
Match location:
Position 1209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAGAAATGCCTCTCTAT
Hairpin Sequence:
5'-CCGG-CAGGAAGAAATGCCTCTCTAT-CTCGAG-ATAGAGAGGCATTTCTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAGAAATGCCTCTCTATCTCGAGATAGAGAGGCATTTCTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAGAAATGCCTCTCTATCTCGAGATAGAGAGGCATTTCTTCCTG-3'

Other clones with same target sequence:

(none)