Construct: shRNA TRCN0000201522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026004.1-540s1c1
DNA Barcode:
CGTTATGCTCAAGGAAGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nt5c3 (107569)
Gene Description:
5'-nucleotidase, cytosolic III
Transcript:
RefSeq NM_026004.1 (NON-CURRENT)
Match location:
Position 540 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATGCTCAAGGAAGGATA
Hairpin Sequence:
5'-CCGG-CGTTATGCTCAAGGAAGGATA-CTCGAG-TATCCTTCCTTGAGCATAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATGCTCAAGGAAGGATACTCGAGTATCCTTCCTTGAGCATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATGCTCAAGGAAGGATACTCGAGTATCCTTCCTTGAGCATAACG-3'

Other clones with same target sequence:

  1. TRCN0000292921