Construct: shRNA TRCN0000201572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172417.1-2485s1c1
DNA Barcode:
CAGCGGAACATCTTAGCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Perm1 (74183)
Gene Description:
PPARGC1 and ESRR induced regulator, muscle 1
Transcript:
RefSeq NM_172417.1 (NON-CURRENT)
Match location:
Position 2485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCGGAACATCTTAGCTTAA
Hairpin Sequence:
5'-CCGG-CAGCGGAACATCTTAGCTTAA-CTCGAG-TTAAGCTAAGATGTTCCGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCGGAACATCTTAGCTTAACTCGAGTTAAGCTAAGATGTTCCGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCGGAACATCTTAGCTTAACTCGAGTTAAGCTAAGATGTTCCGCTG-3'

Other clones with same target sequence:

(none)